Regulatory

Part:BBa_K3857000:Design

Designed by: Luis Arturo Garcia Cruz   Group: iGEM21_RUM-UPRM   (2021-10-20)


algD promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 646
    Illegal SpeI site found at 399
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 646
    Illegal SpeI site found at 399
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 646
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 646
    Illegal SpeI site found at 399
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 646
    Illegal SpeI site found at 399
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to codon-optimize the promoter for E. coli DH5alpha.


Source

the source of this part is derived from the genomic sequence of P. aeruginosa spp.

References